Human LA / SS-B (Recombinant)

Recombinant Human LA/SS-B

Source: E.Coli

Background: LA/SS-B (SS-B Antigen) is a cytoplasmic or nuclear ribonucleoproteins carrying LA determinant.SS-B/La is a nuclear protein of 48 kilodaltons with two structural domains of Mr 28,000 and Mr 23,000 generated by proteolytic cleavage.The human La (SS-B) autoantigen is an abundantly expressed putative RNA chaperone, functioning in various intracellular processes involving RNA.

Description: Recombinant Human LA/SS-B produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 47.6kDa. Recombinant LA/SS-B is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered clear solution.

Formulation: Recombinant Human LA/SS-B ,at a concentration of 1.6mg/ml contains 10mM Sodium Phosphate, 150mM NaCl and 0.1% NaN3 (pH: 7.2).

Stability: Recombinant LA/SS-B although stable at 4oC for 3 weeks, should be stored desiccated below -18 C. Please avoid freeze-thaw cycles.

Purity: Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of LA/SS-B

Protein content: Protein quantitation was carried out by using: 0.25 - 2.0 mg/ml Bradford assay vs. BSA

Usage: BioWORLD\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.