Human L-Selectin (Recombinant)

Recombinant Human L-selectin

Source: E.Coli

Description: L-Selectin belongs to a family of divalent cation-dependent carbohydrate-binding glycoproteins or adhesion molecules. Lselectin is expressed constitutively on lymphocytes, monocytes and granulocytes and interacts specifically with carbohydrate groups on activated endothelial cells. L-Selectin may be shed by proteolytic cleavage and circulating levels in biological fluids may be used as an indicator of various pathological conditions. Recombinant Human L-Selectin is expressed in E. coli containing 294 amino acids 39-332 fused to an amino terminal hexahistidine tag.

Physical Appearance: Sterile Filtered clear soultion.

Formulation: Recombinant LSel is supplied in 1x Laemmli Buffer (25mM Tris-HCl pH6.8, 50mM DTT, 1%(w/v) SDS, 0.1%(w/v) Bromophenol Blue and 2.5% Glycerol).

Stability: Store at 4 C if entire vial will be used within 1-2 weeks. Store, frozen at -20 C for longer periods of time. Please avoid freeze-thaw cycles.

Applications: Recombinant Human L-Selectin can be used directly as a positive control in Western blotting, ELISA, immunoprecipitation and other immunological experiments. The biological activity of this product has not yet been tested.

Purity: Greater than 95% (SDS-PAGE). Single band on Western Blot.

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.