Human Lymphotactin (XCL1), (Recombinant)

Recombinant Human Lymphotactin (XCL1)

Source: E.Coli

Description: Recombinant Human Lymphotactin produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 92 amino acids and having a molecular mass of 10007 Dalton. Human Lymphotactin is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Human Lymphotactin was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.

Solubility: It is recommended to reconstitute the lyophilized Human Lymphotactin in sterile 18MO-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized Human Lymphotactin although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Human Lymphotactin should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 99.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Ser-Glu-Val-Ser.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: bioWORLD\'s Lymphotactin is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract human T cells at 10.0-100.0 ng/ml.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Lymphotactin.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC, using a calibrated solution of Lymphotactin as a Reference Standard.

Usage: bioWORLD\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.