Human Matrix MetalloProteinase-13 (Recombinant)

Recombinant Human Matrix Metalloproteinase-13 (MMP-13)

Source: E.Coli

Background:

Description: Latent recombinant human pro-collagenase (MMP-13) also called collagenase-3 truncated from C-terminal. Recombinant Human MMP-13 produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 27kDa. Recombinant MMP-13 is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered clear solution.

Formulation: The protein Solution (100 g/ml, 0.1 ml) in 0.05M Tris-HCl buffer, pH 7.5, containing 0.2M NaCl, 5mM CaCl2, 20 M ZnSO4 and 0.1% BSA.

Stability: Recombinant MMP-13 although stable at 140C for 1 week, should be stored desiccated below -180C. Please avoid freeze-thaw cycles.

Purity: Greater than 90% as determined by SDS-PAGE.

Biological Activity: ProSpec\\\\\\\'s Recombinant MMP-13 activity is determined by the cleavage of fluorogenic peptide, 100 ng of enzyme activated with APMA will digest 80% fluorogenic peptide substrate (0.1ml of 20 M solution) at 35 C for 30 minutes, (10 g=100 assays).

Applications: Used as a standard for assaying MMP-13 or for screening inhibitors.

Usage: BioWORLD\\\\\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.