Human Matrix MetalloProteinase-3 (Recombinant)

Recombinant Human Matrix Metalloproteinase-3 (MMP-3 )

Source: E.Coli

Background: Matrix metalloproteinases are important regulators of extracellular matrix molecules and cell-cell signaling and are involved in the regulation of the pericellular space, including the cleavage of matrix proteins. In particular, MMP3 has been shown to be effective in cleaving heparan sulfate proteoglycans. Purified recombinant MMP3 can directly cleave agrin, and it can also remove agrin from synaptic basal lamina. MMP3 activity is itself regulated as activation of MMP3 is lost in denervated muscles.

Description: Recombinant Human MMP-3 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 230 amino acids and having a molecular mass of 22kDa. Matrix Metalloproteinase-3 is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with no additives.

Stability: Lyophilized recombinant Human MMP-3 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution rHuMMP-3 should be stored at 40C between 2-7 days and for future use below -180C. Please avoid freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: ProSpec\'s MMP-3 is fully biologically active when compared to standards. The specific activity was found to be =1,250 mU/mg protein (H. Nagase, et al; J. Biol. Chem. 269, 20952, 1994). One unit is defined as the amount of enzyme that hydrolyzes 1 mol of MCA-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(DNP)-NH2 per min. at 37 C, pH 7.0.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Recombinant MMP-3 .

Usage: BioWORLD\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.