Human Matrix Metalloproteinase-8 (Recombinant)

Recombinant Human Matrix Metalloproteinase-8

Source: E.Coli

Description: Full-length recombinant human neutrophil pro-collagenase (MMP-8), latent form. Recombinant Human MMP-8 produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 75kDa. Recombinant MMP-8 is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered clear solution.

Formulation: The protein Solution (100 units/ml) in 0.05M Tris-HCl buffer, pH 7.6, containing 0.2M NaCl, 5mM CaCl₂, 0.0025% NaN₃ and 0.1% BSA.

Stability: Recombinant MMP-8 although stable at 14°C for 1 week, should be stored desiccated below -18°C. Please avoid freeze-thaw cycles.

Purity: Greater than 90% as determined by SDS-PAGE.

Unit definition: One unit of collagenolytic activity is defined as the cleavage of 1 μg of collagen per minute.

Biological Activity: 100 units/ml after activation with APMA by solution assay method.
One unit of collagenolytic activity is defined as the cleavage of 1µg of collagen per minute by the solution method.

Applications: Used as a standard for analyzing mammalian collagenase activity.

Usage: BioWORLD\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.