Human Monocyte Chemotactic Protein-4 (CCL13) (Recombinant)

Recombinant Human Monocyte Chemotactic Protein-4 (CCL13) (rHuMCP-4)

Source: E.Coli

Description: Recombinant Human MCP-4 produced in E.Coli is a non-glycosylated, Polypeptide chain containing 74 amino acids and having a molecular mass of 8510 Dalton. Human Monocyte Chemotactic Protein is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.

Solubility: It is recommended to reconstitute the lyophilized MCP-4 in sterile 18MO-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized Monocyte Chemotactic Protein-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Monocyte Chemotactic Protein-4 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ala-Leu. .

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: bioWORLD\'s MCP-4 is fully biologically active when compared to standard. The specific activity as determined by the ability of MCP-4 to chemoattaract human monocytes at 10-100ng/ml.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Human Monocyte Chemotactic Protein-4.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC, using a calibrated solution of Human MCP-4 as a Reference Standard.

Usage: bioWORLD\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.