Human Neuronal Specific Enolase, His Tag (Recombinant)

Recombinant Human Neuron Specific Enolase (NSE)

Source: E.Coli

Background: NSE is the ?? isoform of the glycolytic enzyme enolase and is expressed primarily in neurons, in normal and neoplastic neuroendocrine cells. NSE is a highly soluble cytoplasmic protein that is readily secreted into the CSF and serum following tissue damage. NSE shows neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons and binds in a calcium-dependent manner to cultured neocortical neurons promoting cell survival.

Description: Recombinant Human NSE is expressed in E. coli containing 433 amino acids 2-434 fused to an amino terminal hexahistidine tag.

Physical Appearance: Sterile Filtered clear soultion.

Formulation: Recombinant NSE is supplied in 1x Laemmli Buffer (25mM Tris-HCl pH6.8, 50mM DTT, 1%(w/v) SDS, 0.1%(w/v) Bromophenol Blue and 2.5% Glycerol).

Stability: Store at 4 C if entire vial will be used within 1-2 weeks. Store, frozen at -20 C for longer periods of time. Please avoid freeze-thaw cycles.

Applications: Recombinant Human NSE can be used directly as a positive control in Western blotting, ELISA, immunoprecipitation and other immunological experiments. The biological activity of this product has not yet been tested.

Purity: Greater than 95% (SDS-PAGE). Single band on Western Blot.

Usage: BioWORLD\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.