Human Neurotrophin-3 (Recombinant)

Recombinant Human Neurotrophin-3 (NT-3)

Source: E.Coli

Background: NT-3 is a neurotrophic factor involved in regulating the survival of visceral and proprioceptive sensory neurons. It is closely homologous to Nerve Growth Factor beta (NGF-b) and BDNF. NT-3 is found in neurons of the central nervous system. NT-3 is expressed also in muscles and its expression is downregulated in denervated muscles. Many human gliomas express and secrete NT-3 into the conditioned medium .

Description: Recombinant Human NT-3 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 119 amino acids and having a molecular mass of 13606.29 Dalton. Recombinant NT-3 is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.

Solubility: It is recommended to reconstitute the lyophilized NT-3 in sterile 18M-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized NT-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution NT-3 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Tyr-Ala-Glu-His-Lys.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: NT-3 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant induction of choline acetyl transferase in rat basal forebrain primary septal culture was found between 20-50 ng/ml.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of NT-3.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 2.165 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of NT-3 as a Reference Standard.

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.