Human Neutrophil Activating Protein-2 (CXCL7), (Recombinant)

Recombinant Human Neutrophil Activating Protein-2 (CXCL7) (NAP-2)

Source: E.Coli

Description: Recombinant Human NAP-2 produced in E.Coli is a non-glycosylated, Polypeptide chain containing 70 amino acids and having a molecular mass of 7609 Dalton. Recombinant NAP-2 is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Recombinant Neutrophil-activating peptide 2, lyophilized from a concentrated (1 mg/ml) solution in water containing no additives.

Solubility: It is recommended to reconstitute the lyophilized rHuMCP-1 in sterile 18MO-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized NAP-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution NAP-2 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Glu-Leu-Arg-Cys

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: bioWORLD's NAP-2 is fully biologically active when compared to standard. The specific activity as determined by the ability of NAP-2 to chemoattract human neurotrophils using a concentration of 1-10 ng/ml.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of NAP-2.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC, using a standard solution of NAP-2 as a Reference Standard.

Usage: biWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.