Human Omentin (Recombinant)

Recombinant Human Insulin Like Growth Factor Binding Protein-2 (IGFBP-2)

Source: E.Coli

Background: Insulin Like Growth Factor Binding Protein-2 is one of the six homologous proteins that specifically bind insulin-like growth factors and modulate their mitogenic and metabolic actions. IGFBP-2 is observed mainly in brain and liquor, showing complex patterns of gene expression during postnatal brain development. Elevated levels IGFBP-2 have been observed in the serum of prostate cancer patients. IGFBP-2 expression does not depend on Growth hormone .

Description: Recombinant bovine IGFBP-2 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 284 amino acids and having a molecular mass of 30776 Dalton. Recombinant bovine IGFBP-2 is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: IGFBP-2 was lyophilized with no additives.

Solubility: It is recommended to reconstitute the lyophilized bovine IGFBP-2 in sterile 18MO-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized IGFBP-2 although stable at room temperature for 2 weeks, should be stored desiccated below -18 C. Upon reconstitution bovine IGFBP-2 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: Insulin Like Growth Factor Binding Protein-2 is fully biologically active when compared to standards. The ED50, calculated by IGF-I Binding Assay was found to be less then 10 nM.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of IGFBP-2 .

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a standard solution of IGFBP-2 as a Reference Standard.

Usage: BioWORLD\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.