Human Parathyroid Hormone (1-34), (Recombinant)

Recombinant Human Parathyroid Hormone (rHuPTH)

Source: E.Coli

Description: Recombinant Human PTH produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 34 amino acids and having a molecular mass of 4117.8 Dalton. rHuPTH is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: The protein (1mg/ml) was lyophilized after extensive dialyses against 1.15mg sodium citrate, sodium chloride 7.31mg, 0.21mg citric acid, 0.1117 EDTA-Na2, 0.2mg Tween 80 and 50mgs Mannitol.

Solubility: It is recommended to reconstitute the lyophilized rHuPTH in sterile 18MO-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized rHuPTH although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rHuPTH should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Amino-Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Val-Ser-Glu-Ile.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: ProSpec\'s rHuPTH is fully biologically active when compared to standards. The activity calculated by UMR106 cell/cAMP method corresponding to a specific activity of 1.0 x 104 Units/mg.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of rHuPTH.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a calibrated solution of PTH as a Reference Standard.

Usage: BioWORLD\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.