Human Peroxisome Proliferator Activated Receptor Gamma (Recombinant)

Recombinant Human Peroxisome Proliferator Activated Receptor Gamma (PPAR-g)

Source: E.Coli

Background: The peroxisome proliferator activated receptors (PPARs) are ligandactivated transcription factors within the nuclear receptor superfamily, which play important roles in adipogenesis, glucose homeostasis and inflammation. Three different isotypes can be distinguished; alpha, beta and gamma. PPAR-g, is mainly expressed in adipose tissue and to lesser extent in colon, the immune system and the retina. Whereas PPARa operates in the catabolism of fatty acids in the liver, PPAR gamma influences the storage of fatty acids in the adipose tissue and plays a role in adipocyte differentiation. PPARg binds to DNA as a heterodimer with retinoid X receptor (RXR), activating expression of target genes after binding ligand. Recombinant Human Peroxisome Proliferator Activated Receptor Gamma is expressed in E. coli having a molecular weight of 59.2 kDa and fused to an amino terminal hexahistidine tag.

Description: Recombinant Human PPAR-g is the full-length form of the protein fused with GST and carries a histidine tag on the N-terminus.

Physical Appearance: Sterile Filtered clear soultion.

Formulation: 1x Laemmli Buffer (25mM Tris-HCl pH6.8, 50mM DTT, 1%(w/v) SDS, 0.1%(w/v) Bromophenol Blue and 2.5% Glycerol).

Storage: Store at 4 C if entire vial will be used within 2-4 weeks. Store, frozen at -20 C for longer periods of time. Please avoid freeze-thaw cycles.

Purity: Greater than 95% (SDS-PAGE). Mass spectrometry of tryptic digest products for identity determination.

Usage: BioWORLD\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.