Human Phosphoinositide 3-kinase p110d/p85a (Recombinant)

Recombinant Human Phosphoinositide 3-kinase p110d/p85a (PI3Kd)

Source: Sf9 insect cells.

Description: The class IA PI3Kd is expressed in endothelial and in white blood cells. Recently it was shown that the inactivation of PI3Kd in bone marrow mast cells (BBMCs) leads to defectivem stem cell factor-mediated proliferation, adhesion and migration of these cells, and to impaired allergen-IgEinduced degranulation and cytokine release. In neutrophils a role for PI3Kd in TNFa-induced signalling was demonstrated by a reduction in Aktphosphorylation and PDK1 activity upon treatment with the d-specific inhibitor IC87114. The catalytic subunit carries a GST-Tag which facilitates the protein\'s application in typical GST pull-down assays and the heterodimer was purified by affinity chromatography. The molecular weight for p110d chain is 120.2 kDa (without Tag) and the p85a chain 83.5 kDa.

Physical Appearance: Sterile Filtered clear soultion.

Formulation: 0.5mg/ml solution in 25 mM Tris, pH 8.0, 50 mM NaCl, 0.5 mM MgCl2, and 50% glycerol.

Storage: Recombinant Human PI3Kd although stable at 140C for 1 week, should be stored desiccated below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 95.0% as determined by SDS Page.

Biological Activity: ~ 3 nmol/mg/min using phosphatidylinositol as the substrate.

Usage: BioWORLD\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.