Human Platelet Derived Growth Factor-AA (Recombinant)

Recombinant Human Platelet Derived Growth Factor-AA(PDGF-AA)

Source: E.Coli

Background: PDGF is a mitogenic peptide growth hormone carried in the alpha-granules of platelets. It is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication.The synthesis of PDGF can be induced by Interleukin-1 , Interleukin-6 , TNF-alpha , TGF-beta and EGF

Description: Recombinant Human Platelet Derived Growth Factor-AA is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 125 amino acids and having a total molecular mass of 28511 Dalton. Recombinant PDGF-AA is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: PDGF-AA was lyophilized with no additives.

Solubility: It is recommended to reconstitute the lyophilized PDGF-AA in sterile 18M-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized PDGF-AA although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution PDGF-AA should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Purity: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ile-Glu-Glu-Ala.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: PDGF-AA is fully biologically active when compared to standard. The ED50 calculated by the dose-dependant proliferation of murine BALB/c 3T3 indicator cells (measured by 3H-thymidine uptake) is < 1 ng/ml, corresponding to a Specific Activity of 1 MIU/mg.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of PDGF-AA.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a standard solution of PDGF-AA as a Reference Standard.

Usage: BioWORLD\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.