Human Protein Kinase C q (Recombinant)

Recombinant Human Protein Kinase C theta (PKC-theta)

Source: SF9 insect cells.

Description: Recombinant Human PKC-teta produced in Sf9 is a glycosylated, polypeptide chain containing amino acids 2-706 and having a molecular mass of 86 KD. PKC-t is purified by proprietary chromatographic techniques. This protein is the full-length form of the protein with an amino terminal poly His-tag.

Physical Appearance: Sterile Filtered clear soultion.

Formulation: Recombinant Human PKC-teta is supplied at a of 0.1mg/ml in 10mM Tris, pH 7.4, 0.1M NaCl, 20% glycerol, 1mM DTT, 0.1mM EDTA, 0.2mM PMSF and 0.03% Brij-35.

Unit Definition: ~ 1800 units/mg. One unit of PKCt activity transfers 1 nmole of phosphate to a PKC-t pseudosubstrate peptide per minute at 300C using. Recombinant human active PKC-teta also phosphorylates histone, MARCKS, moesin, RasGRP3, and PKC alpha and PKC epsilon pseudosubstrates. PKC-teta is a novel PKC and does not require calcium for activity. Activity may vary depending on the substrate and reaction conditions. The optimal concentration should be determined for each specific application. PKC-teta is involved in T-cell activation and T-cell receptor signaling including T-cell mediated activation of NF-kB and AP-1.

Storage: Store at 4 C if entire vial will be used within 2-4 weeks. Store, frozen at -20 C for longer periods of time. Please avoid freeze-thaw cycles.

Purity: Greater than 85% (SDS-PAGE).

Usage: BioWORLD\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.