Human Protein Kinase C-e (Recombinant)

Recombinant Human Protein Kinase C epsilon (PKC-e)

Source: SF9 insect cells.

Description: Recombinant Human PKC-epsilon produced in Sf9 is a glycosylated, polypeptide chain containing amino acids 2-737 and having a molecular mass of 87 KD. PKC-e is purified by proprietary chromatographic techniques. This protein is the full-length form of the protein with an amino terminal poly His-tag.

Physical Appearance: Sterile Filtered clear soultion.

Formulation: Recombinant Human PKC-e is supplied at a of 0.1mg/ml in 10mM Tris, pH 7.4, 0.1M NaCl, 20% glycerol, 1mM DTT, 0.1mM EDTA, 0.2mM PMSF and 0.03% Brij-35.

Unit Definition: 3250 units/mg. One unit of PKCe activity transfers 1 nmole of phosphate to a PKCe substrate peptide per minute at 30 C. Recombinant human active PKCe also phosphorylates full length myelin basic protein, MARKS, GAP-34, and raf-1 kinase. Activity requires incubation with lipid activators, and may vary depending on the substrate and reaction conditions. The optimal concentration should be determined for each specific application.

Storage: Store at 4 C if entire vial will be used within 2-4 weeks. Store frozen at -20 C for longer periods of time. Please avoid freeze-thaw cycles.

Purity: Greater than 90% (SDS-PAGE).

Usage: BioWORLD\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.