Human Ribosomal PhosphoProtein P0 (Recombinant)

Recombinant Human Ribosomal Phosphoprotein P0 (RPP0)

Source: Sf9 insect cells.

Background: The ribosomal phosphoproteins(P protein antigens) are associated with the large ribosomal subunit and therefore are antigenic targets with a cytoplasmic localization. Three P proteins have been described: P0 with a molecular weight of 35 kDa, P1 (19 kDa) and P2 (17 kDa). Autoantibodies against ribosomal P proteins are present in 10 % of SLE patients. If anti-ribosomal P antibodies were to occur in the absence of other typical SLE associated autoantibodies, they may account for some patients with so-called ANA-negative lupus. It has been reported that lupus patients positive for anti-ribosomal P autoantibodies have a high frequency of CNS involvement, suggesting a marker use for these antibodies.

Description: Recombinant Human Ribosomal Phosphoprotein P0 produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 35,096 dalton. Recombinant Human Ribosomal Phosphoprotein P0 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered clear solution.

Formulation: Recombinant Human Ribosomal Phosphoprotein P0 is supplied at a of 0.9mg/ml in20mM HEPES buffer pH-7.5, 0.01 mM EDTA. 0.02% SDS.

Stability: Store at 4 C if entire vial will be used within 2-4 weeks. Store, frozen at -20 C for longer periods of time. Please avoid freeze-thaw cycles.

Purity: Greater than 80% (SDS-PAGE).

Immunological functions: 1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels.

Applications: Western-Blot with monoclonal anti-hexa-His-tag antibody & SLE sera (Systemic Lupus Erythematodes Disease).

Coating concentration: 0.3-0.7 g/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.

Usage: BioWORLD\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.