Human Sertuin-6 (Recombinant)

Calpastatin

Source: Human Eryhtrocytes

Physical Appearance: Sterile Filtered colorless solution.

Formulation: 50mM imidazole-HCl, 0.2mM EGTA and 1mM DTT pH-7.4. Tris-HCL and 1mM EGTA.

Stability: Calpastatin although stable at 100C for 1 week, should be stored desiccated below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 90% as determined by: Analysis by reducing and non-reducing SDS-PAGE Coomassie.

Applications: This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection. For adsorption we suggest the following procedure: A-Dilute 1 l of the antiserum against Calpastatin in 1ml of the usual buffer for immunohistochemistry (final dilution 1: 1000). B- Add 1 g of protein to 1ml of the diluted antibody solution and mix well. C-Incubate for at least 6 hours in the cold. D-Apply to tissue-sections and incubate for 3 days. E-Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB). As a result, the immunostaining should be strongly reduced or even completely prevented.

Refrencesy: Lane et al. (1992) A comparison of the intracellular distribution of -calpain, mcalpain and calpastatin in proliferating human A431 cells. Exp. Cell Res. 203: 5-16.

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.