Human Soluble RANK Ligand (Recombinant)

Recombinant Human Soluble RANK ligand

Source: E.Coli

Description: Recombinant Human soluble RANKL produced in E.Coli is a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 20006 Dalton. Recombinant sRANKL is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Recombinant Soluble RANK ligand was lyophilized from a concentrated (1mg/ml) solution containing 10mM Tris, pH-7.6 and 50mM sodium chloride.

Solubility: It is recommended to reconstitute the lyophilized RANKL in sterile 18M?-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized sRANKL although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution RANK ligand should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Lys-Ala-Met.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: RANK ligand is fully biologically active when compared to standard. The activity is determined by its ability to induce osteoclast formation in RAW264.7 cells using a conc. of 5.0-10.0 ng/ml and by dose dependant stimulation of IL-8 production in human PBMC which was found to be <10 ng/ml.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of hIL-20.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a calibrated solution of Soluble RANK ligand as a Reference Standard.

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.