Human Thyroid Peroxidase, (Recombinant)

Recombinant Human Thyroid Peroxidase (TPO)

SourceSf9 insect cells.

Background Thyroid Peroxidase (TPO) represents one of the main autoantigenic targets in autoimmune thyroid disease of humans. Its identity with the formerly so-called 'microsomal antigen' has been shown several years ago. As an integral membrane glycoprotein it is restricted to the apical plasma membrane of the follicular epithelial cells and comprises two identical subunits of approx. 100 kDa molecular weight. The hemoprotein TPO plays a key role in the thyroid hormone biosynthesis by catalysing both the iodination of tyrosyl residues and the coupling of iodotyrosyl residues in thyroglobulin (TG) to form precursors of the thyroid hormones T4 and T3.

Description Recombinant Human TPO produced in SF9 is a glycosylated, polypeptide chain coding for the extracellular domain of native TPO, containing 834 amino acids and having a molecular mass of 92,872 dalton. Recombinant TPO is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Formulation Recombinant Human TPO is supplied at a of 0.5mg/ml in 16mM HEPES pH-7.6, 160mM NaCl, 0.08mM Kl and 20% glycerol.

Immunological functions 1. Binds IgG-type human auto-antibodies. 2. Auto-antibodies to TPO recognize conformation-dependent epitopes. 3. Standard ELISA test (checker-board analysis of positive/negative sera panels, including international reference serum NIBSC 66/387).

Applications Western-Blot with monoclonal anti-TPO antibody and monoclonal anti-hexa-His-tag antibody.

Coating concentration 0.15-0.375 g/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.

Storage Conditions Store at 4 C if entire vial will be used within 2-4 weeks. Store, frozen at -20 C for longer periods of time. Please avoid freeze-thaw cycles.

Purity Greater than 95% (SDS-PAGE).

Usage BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.