Human Transforming Growth Factor-Beta 3 (Recombinant)

Recombinant Human Transforming Growth Factor-beta 3

Source: E.Coli

Background: Transforming growth factor-betas (TGF betas) are multifunctional peptides that regulate growth and differentiation in a variety of cells. The transforming growth factor beta (TGF-beta) family of growth modulators play critical roles in tissue development and maintenance. Recent data suggest that individual TGF-beta isoforms (TGF-beta 1, -beta 2 and -beta 3) have overlapping yet distinct biological actions and target cell specificities, both in developing and adult tissues Transforming growth factor (TGF) beta 3 is a new isoform of the TGF beta superfamily and is presumed to play an important role in wound repair and scarring. TGF-beta 3 is believed to be involved in osteoblast proliferation, chemotaxis, and collagen synthesis.

Description: Recombinant Human TGF-b 3 produced in E.Coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide chain containing 112 amino acids and having a molecular mass of 25,445 Dalton. Recombinant Human TGF-b 3 is purified by standard chromatographic techniques.

Physical Appearance: Sterile Filtered clear solution.

Formulation: The protein solution (0.25mg/ml) contains 20% ethanol and 0.1% acetate.

Stability: Recombinant Human TGF-beta 3 although stable at room temperature for 3 weeks, should be stored at 40C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Purity: Greater than 99.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Leu-Asp-Thr-Asn.

Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: ProSpec\\\'s TGF-beta 3 is fully biologically active when compared to standard. The ED50 as determined by the cell toxicity assay using the WHO Standard 98/608 as a direct comparison is < 0.05ng/ml, corresponding to a Specific Activity of 12.5 x 106IU/mg.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Recombinant Human TGF-beta 3.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.718 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of TGF-beta 3 as a Reference Standard.

Usage: BioWORLD\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.