Human Tumour Type M2 Pyruvate Kinase (Recombinant)

Recombinant Human Tumor Type M2 Pyruvate Kinase (PKM2)

Source: E.Coli

Description: Pyruvate kinase is a key enzyme in the glycolytic pathway. The M2 isoenzyme of pyruvate kinase is specifically expressed at high levels in tumour cells, and can be measured in plasma of patients with advanced breast cancer. The marker is useful for measuring disease activity, sensitivity to chemotherapy and recurrence.

Description: Recombinant Human Tumor Type M2 Pyruvate Kinase is expressed in E. coli having a molecular weight of 62.4 kDa fused to an amino terminal hexahistidine tag.

Physical Appearance: Sterile Filtered clear soultion.

Formulation: Recombinant Human PKM2is supplied in 1x Laemmli Buffer (25mM Tris-HCl pH6.8, 50mM DTT, 1%(w/v) SDS, 0.1%(w/v) Bromophenol Blue and 2.5% Glycerol).

Application: Recombinant PKM2 can be used directly as a positive control in Western blotting, ELISA, immunoprecipitation and other immunological experiments. The biological activity of this product has not yet been tested.

Storage: Store at 4 C if entire vial will be used within 1-2 weeks. Store, frozen at -20 C for longer periods of time. Please avoid freeze-thaw cycles.

Purity: Greater than 95% (SDS-PAGE).

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.