Human Ubiquitin Carboxyl-Terminal Hydrolase L1 GST Tag (Recombinant)

Recombinant Human Protein Gene Product 9.5 (PGP9.5)

Source: E.Coli

Background: Protein gene product-9.5(PGP9.5) ,known as ubiquitin carboxyl-terminal esterase , is a neuron specific protein, structurally and immunologically distinct from neuron specific enolase. The protein which has a molecular weight of 27kDa was first identified by high resolution two dimensional PAGE. Standard immunohistochemical techniques have demonstrated the presence of PGP9.5 in neurons and nerve fibres at all levels of the central and peripheral nervous system, in many neuroendocrine cells, in segments of the renal tubules, in spermatogonia and Leydig cells of the testis, in ova and in some cells of both the pregnant and non-pregnant corpus luteum.

Description: Recombinant PGP-9.5 shows a band at 36kDa on SDS-PAGE commassie blue stained gel. Recombinant PGP9.5 is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile filtered liquid.

Formulation: 100 g/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA, 20% Glycerol without BSA and Sodium Azide.

Stability: Store vial at -20Co to -80Co. When stored at the recommended temperature, recombinant PGP9.5 is stable for 12 months. Please avoid freeze-thaw cycles.

Purity: Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE coomassie blue gel.

Dimers and aggregates: Less than 1% as determined by coomassie blue SDS-PAGE gel analysis.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Human PGP-9.5.

Usage: BioWORLD\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.