Human Ubiquitin (Recombinant)

Recombinant Human Ubiquitin (Ub)

Source: E.Coli

Background: Recombinant Human Ubiquitin (Ub) is a small protein composed of 76 amino acids and having a molecular mass of 8.6kDa. Ub is found only in eukaryotic organisms among which it shows a strong sequence conservation. The protein is present in all cell types, thus giving rise to its name. Ub is found either in free form or conjugated to proteins through a covalent bond between the glycine at the C-terminal end of Ub and the side chains of lysine on the proteins. The attachment of multiple copies of ubiquitin targets the proteins for degradation by the 26S proteosome. Ubiquitin ligation is an ATP-dependent multi-step process: Ub is activated by the E1 enzyme, the attachment of Ub to the target protein is catalyzed by the E2 enzyme acting in concert with E3 which plays a role in recognizing the substrate protein.

Description: Recombinant human ubiquitin expressed in Escherichia coli and purified by ion-exchange chromatography.

Physical Appearance: Sterile filtered liquid.

Formulation: 1mg/ml containing 50mM HEPES (pH7.5) 150mM NaCl, 1mM DTT and 10%glycerol.

Stability: Store at -20 C if intended use is longer than 1 week, store at 4 C for a period up to 1 week. Please avoid freeze-thaw cycles.

Purity: minimum 95% as estimated by analysis of Coomassie blue stained SDS-PAGE.

Sequence: MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

Selected Application References: Haas AL, Siepmann TJ (1997) Pathways of ubiquitin conjugation. FASEB J. 11(14): 1257-68. Hershko A, Ciechanover A (1998) The ubiquitin system. Annu Rev Biochem. 67: 425-79. Pickart CM (2000) Ubiquitin in chains. Trends Biochem Sci. 25(11): 544-8. Pickart CM, Eddins MJ (2004) Ubiquitin: structures, functions, mechanisms. Biochim Biophys Acta. 1695(1-3): 55-72. Roos-Mattjus P, Sistonen L (2004) The ubiquitin-proteasome pathway. Ann Med. 36(4): 285-95.

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.