Listeriolysin-O PEST-Free (Recombinant)

Recombinant Listeriolysin-O PEST free (LLO-PEST)

Source: E.Coli

Background: The secreted pore-forming protein listeriolysin O (LLO) is an essential virulence determinant of the facultative intracellular bacterium Listeria monocytogenes that allows the pathogen to escape from the phagocytic vacuole and reach the host cytosol. LLO is a single polypeptide protein encoded by the hlyA gene and composed of 529 residues. At its NH2-terminus it possesses a 25 residues long typical signal sequence excited during the secretion process. Moreover, in its NH2-terminus there is also a 19 amino acids PEST-like sequence that may target this toxin for degradation. This motif seems to be essential for bacterial virulence too, since its deletion causes a defect in bacterium phagosomal escaping.

Description: Recombinant Listeriolysin O lacking both the signal secretion sequence and the PEST- like sequence. LLO-PEST minus is composed of 471 amino acids, starting from amino acid 60 to amino acid 529, with the addition of a methionine in its NH2-terminus.

Physical Appearance: Sterile Filtered clear solution.

Formulation: The protein (0.2mg/ml) contains 50mM NaH2PO4, 1mM EDTA, 2.7mM KCl, 1mM DTT, 5% glycerol and 0.5M NaCl.

Stability: rLLO although stable at 140C for 1 week, should be stored desiccated below -180C. Please prevent freeze-thaw cycles. Please avoid freeze-thaw cycles.

Purity: Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel

Activity: 1.25x106 HU/mg. 2 mM DTT could be use to reactivate the toxin.

Endotoxin: Less than 0.1ng/ g (IEU/ g) of LLO.

Applications: a. Cytosolic delivery of molecules, peptides, oligonucleodides and plasmid DNA (4). b. Production of specific monoclonal antibodies (5). c. Detection of anti-listeriolysin O antibodies (6).

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.