Listeriolysin-O (Recombinant)

Recombinant Listeriolysin-O (LLO)

Source: E.Coli

Background: Recombinant Listeriolysin O (LLO) is a single polypeptide protein secreted by the Gram-positive bacterium Listeria monocytogenes. LLO belongs to the group of cholesterol-binding sulfhydrylactivated toxins, the lytic activity of which is enhanced by reducing agents and is suppressed by exposure to oxygen or cholesterol. LLO hemolytic activity is maximum at pH 5.5 and rapidly decreases with the increase of the pH. Listeriolysin O is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered clear solution.

Formulation: The protein (0.5mg/ml) contains 50mM NaH2PO4, 1mM EDTA, 2.7mM KCl, 1mM DTT, 5% glycerol and 0.5M NaCl.

Stability: Store at refrigerated if the entire vial will be used within 2-4 days. Please avoid freeze-thaw cycles.

Purity: Greater than 90.0% as analysed by SDS-PAGE.

Biological Activity: Recombinant LLO has a hemolytic activity 1.25x106 HU/mg. 2mM DTT is used to reactivate the toxin.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of LLO.

Applications: a. Cytosolic delivery of molecules, peptides, oligonucleodides and plasmid DNA. b. Production of specific monoclonal antibodies. c. Detection of anti-listeriolysin O antibodies.

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.