Malate Dehydrogenase (Recombinant)

Malate(Malic) Dehydrogenase (MDH1), Chicken recombinant

Source: E. Coli

Background: Malate Dehydrogenase is an enzyme that catalyzes the conversion of (S)-malate and NAD+ to oxaloacetate and NADH. Recombinant MDH is purified by proprietary chromatographic techniques.

Description: The DNA encoding Malate (Malic) Dehydrogenase is cloned from cDNA library of chicken heart.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Malate(Malic) Dehydrogenase lyophilized from a sterile solution at a concentration of 1 mg/ml with 0.59 mg/ml Sodium phosphate.

Solubility: It is recommended to reconstitute the lyophilized Malate Dehydrogenase in sterile 18MO-cm H2O.

Stability: Lyophilized MDH although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Recombinant MDH can be stored at 4°C for 2-7 days and at -18°C for longer. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE .

Unit definition: One unit is defined as 1 μmol of NAD+ production per minute under the assay conditions (25°C, pH 7.5).

Specific Activity: Greater than 710U/mg protein.

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.