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Description

Recombinant Murine Calretinin

Source: E.Coli

Background: Calretinin is an intracellular calcium-binding protein belonging to the troponin C superfamily characterized by a structural mitif described as the EF-hand domain. The immunohistochemical detection of calretinin in developing cerebellum is restricted to the later stages indicated by weak staining from week 21 of gestation, in Purkinje and basket cells and in neurons of the dentate nucleus. The intensity of staining increases as the cerebellum matures. In tumors, calretinin has been detected in mesotheliomas and some pulmonary adenocarcinomas.

Description: Recombinant Calretinin shows a band at 57kDa on SDS-PAGE commassie blue stained gel. Recombinant Murine Calretinin protein is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile filtered liquid.

Formulation: 100 g/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA, 20% Glycerol without BSA and Sodium Azide.

Stability: Store vial at -20Co to -80Co. When stored at the recommended temperature, Murine Calretinin protein is stable for 12 months. Please avoid freeze-thaw cycles.

Purity: Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE coomassie blue gel.

Dimers and aggregates: Less than 1% as determined by coomassie blue SDS-PAGE gel analysis.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Murine Calretinin.

Usage: BioWORLD\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Properties

Storage Temperature

-20°C