Mouse Fibroblast Growth Factor-9 (Recombinant)

Recombinant murine Fibroblast Growth Factor-9 (FGF-9)

Source: E.Coli

Description: Recombinant Murine FGF-9 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 205 amino acids and having a molecular mass of 23308 Dalton. Recombinant FGF9 is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing 20mM Tris pH=8 and 75mM NaCl.

Solubility: It is recommended to reconstitute the lyophilized FGF9 in sterile 18MO-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized FGF-9 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution FGF9 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence: he sequence of the first five N-terminal amino acids was determined and was found to be Pro-Leu-Gly-Glu-Val.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: rmFGF-9 is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <0.5 ng/ml, corresponding to a specific activity of 2 x 106 Units/mg.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Recombinant Human Fibroblast Growth Factor-basic.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC, using a standard solution of FGF9 as a Reference Standard.

Usage: BioWORLD\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.