Mouse GRO/KC (CXCL1), (Recombinant)

Recombinant Mouse GRO1/N51/KC (CXCL1)

Source: Escherichia Coli

Description: Recombinant murine KC also nown as N51 and GRO-1 produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of 8kDa.Recombinant Murine GRO is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: The protein was lyophilized with no additives.

Solubility: It is recommended to reconstitute the lyophilized Recombinant GRO1 in sterile 18MO-cm H₂O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized Murine N51/KC although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GRO1 can be stored 2-7 days at 4°C, for longer storage, store below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Amino acid sequence: RLATGAPIANELRCQ CLQTMAGIHL KNIQSLKVLP SGPHCTQTEV IATLKNGREA CLDPEAPLVQ KIVQKMLKGV PK

Biological Activity: The biological activity was determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human neutrophils. Significant calcium mobilization is observed with 50ng/mL of recombinant mouse KC (Specific Activity: 2 x 10000 units/mg).

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.03 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of KC as a Reference Standard.

Usage: For LABORATORY RESEARCH USE ONLY.