Mouse Insulin Like Growth Factor-1 (Recombinant)

Recombinant Murine Insulin Like Growth Factor-I (IGF-1)

Source: E.Coli

Background: A well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on Somatotropin. It is believed to be mainly active in adults in contrast to Insulin like Growth Factor 2, which is a major fetal growth factor.

Description: Recombinant murine IGF-1 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7600 Dalton. IGF-I is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Recombinant Murine IGF-1 was lyophilized after extensive dialysis against 0.2 m-filtered solution in PBS.

Solubility: It is recommended to reconstitute the lyophilized Murine IGF-I in sterile 18MO-cm H₂O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized Murine IGF-I although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Murine IGF-1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by r SDS-PAGEl.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Pro-Glu-Thr-Leu. N-terminal methionine has been completely removed enzymatically.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: IGF-I is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of murine BALBC 3T3 cells (measured by 3H-thymidine uptake) is less then 1.0 ng/ml, corresponding to a specific activity of 1x10⁵ U/mg.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IGF-I as a Reference Standard.

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