Mouse Interleukin-6 (Recombinant)

Recombinant Murine Interleukin-6

Source: E.Coli

Background: Interleukin (IL)-6 is an important proinflammatory and immunoregulatory cytokine expressed by various cells.Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro.

Description: Recombinant Murine IL-6 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21709 Dalton.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution containg 50 mM NH4HCO3, pH 8.0.

Solubility: It is recommended to reconstitute the lyophilized rmIL-6 in 100 mM acetic acid to 0.1-1.0 mg/mL to regain full activity, and can then be further diluted to other aqueous solutions.

Stability: Lyophilized IL-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution IL-6 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ser.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: The ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is less then 0.02 ng/ml, corresponding to a Specific Activity of 1 x 10 7IU/mg.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of IL-6.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a standard solution of IL-6 as a Reference Standard.

Usage: BioWORLD\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.