Mouse Interleukin-7 (Recombinant)

Recombinant Mouse Interleukin-3

Source: E.Coli

Background: Interleukin 7 (IL-7), which is constitutively produced particularly by stromal cells from the bone marrow and thymus, plays a crucial role in T cell homeostasis. This cytokine is implicated in thymopoiesis since it sustains thymocyte proliferation and survival. It regulates peripheral naive T cell survival by modulating the expression of the anti-apoptotic molecule Bcl-2, and sustains peripheral T cell expansion in response to antigenic stimulation. Infection by the human immunodeficiency virus (HIV) leads to severe T lymphopenia and general immune dysfunction. Increased IL-7 plasma levels are generally observed in HIV-infected patients

Description: Recombinant Murine IL-7 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 14897 Dalton. Recombinant Mouse IL-7 is purified by proprietary chromatographic techniques

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.

Solubility: It is recommended to reconstitute the lyophilized Murine IL-7 in sterile 18M?-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized Recombinant Murine IL-7 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Recombinant Murine IL-7 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Cys-His-Ile.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: ProSpec\\\\\\\\\\\\\\\'s Murine IL-7 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of murine 2E8 cells is < 0.2ng/ml, corresponding to a Specific Activity of 2 x 106 IU/mg.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Murine IL-7.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.48 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of Murine IL-7 as a Reference Standard.

Usage: BioWORLD\\\\\\\\\\\\\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.