Mouse Macrophage Inflammatory Protein-1 beta (CCL4), (Recombinant)

Recombinant Murine Macrophage Inflammatory protein-1 beta (CCL4)

Source: E.Coli

Description: Recombinant Murine Macrophage Inflammatory Protein-1 beta produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7808 Daltons. Murine MIP-1b is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Lyophilized from a concentrated (1mg/ml) solution containing no additives

Solubility: It is recommended to reconstitute the lyophilized MIP-1beta in sterile 18MO-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized MIP-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution MIP-1beta should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Gly-Ser.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: bioWORLD\'s Murine Macrophage Inflammatory Protein-1 beta is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract Human blood monocytes at 20-100 ng/ml.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Recombinant Murine MIP-1beta.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC, using a calibrated solution of Recombinant Murine MIP-1b as a Reference Standard.

Usage: bioWORLD\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.