Mouse Monocyte Chemotactic Protein-1 (CCL2), (Recombinant)

Recombinant Murine JE/MCP-1 (CCL2)

Source: E.Coli

Description: Recombinant Murine JE(MCP-1) produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 125 amino acids and having a molecular mass of 13803 Dalton. Murine JE(MCP-1) is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Murine JE(MCP-1) was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.

Solubility: It is recommended to reconstitute the lyophilized Murine JE(MCP-1) in sterile 18MO-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized Murine JE(MCP-1) although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Murine JE(MCP-1) should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 99.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ala-Val.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: bioWORLD\'s Murine JE(MCP-1)is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract Balb/C mouse spleen MNCs at 1.0-20.0 ng/ml.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Murine JE(MCP-1).

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC, using a calibrated solution of Murine JE(MCP-1) as a Reference Standard.

Usage: bioWORLD\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.