Mouse Thrombopoietin (Recombinant)

Recombinant mouse Thrombopoietin (TPO)

Source: E.Coli
Synonym: Megakaryocyte colony-stimulating factor, Myeloproliferative leukemia virus oncogene ligand, C-mpl ligand, ML, Megakaryocyte growth and development factor, MGDF, TPO, MKCSF, MPLLG, MGC163194, THPO.

Description: Recombinant Murine TPO produced in E.Coli is a single, non-glycosylated soluble polypeptide chain containing 174 amino acids and having a molecular mass of 18704 Dalton which comprises the receptor binding domain of the Mpl-ligand protein. Recombinant mouse Thrombopoietin is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.

Solubility: It is recommended to reconstitute the lyophilized Recombinant TPO in sterile 18MO-cm H₂O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized Recombinant TPO although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Recombinant TPO can be stored at 4°C for 2-7 days and below -18°C for longer. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: (a) Analysis by SDS-PAGE.

Amino acid sequence: SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKF.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: Recombinant TPO is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of MO7e cells is less then 1 ng/ml, corresponding to a Specific Activity of 1 x 10⁶ IU/mg.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of TPO.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a calibrated solution of TPO as a Reference Standard.

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.