Mouse Tumor Necrosis Factor-alpha (Recombinant)

Recombinant murine Tumor Necrosis Factor-alpha (rmTNF-a) Certificate of Analysis and Data Sheet

Source: Streptomyces Lividans

Description: Recombinant murine TNF-alpha produced in S.Lividians is a single, non-glycosylated, polypeptide chain containing 156 amino acids and having a molecular mass of 17301.32 Dalton. The rmTNF-alpha is purified by standard chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Each mg contains 50mM Tris-HCL pH-8 and 20mM NaCL.

Solubility: It is recommended to reconstitute the lyophilized rmTNF-a in sterile 18M-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized rmTNF-a although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rmTNF-a should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Ser-Ser.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: rmTNF-alpha is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less then 0.05ng/ml, corresponding to a Specific Activity of 9x108 IU/mg.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of rmTNF-a.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a calibrated solution of TNF- as a Reference Standard.

Usage: BioWORLD\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.