Mycobacterium Tuberculosis major secretory protein Antigen 85B, Recombinant

Synonyms

Antigen 85-B, 85B, Extracellular alpha-antigen, Antigen 85 complex B, Ag85B, Mycolyl transferase 85B, EC 2.3.1.-, Fibronectin-binding protein B, 30 kDa extracellular protein, fbpB, A85B, Major Secretory Protein Antigen 85B.

Antigen 85B Mycobacterium Tuberculosis-is the most abundant protein exposed by M. tuberculosis, as well as a potent immunoprotective antigen and a leading drug target. Ag85 induces strong T-cell proliferation and IFN-g secretion in most healthy individuals exposed to M. tuberculosis, in BCG-vaccinated mice and humans, whereas the antibody against Ag85 are more prevalent in active tuberculosis patients with decreased cellular immune response.

Description

Ag85B Recombinant His-Tag fusion protein (1-325 a.a.) produced in E. coli is a single, non-glycosylated polypeptide chain having a molecular mass of 30kDa.

Source

Escherichia coli

Physical Appearance

Sterile Filtered and lyophilized, though might appear as a solution as a result of the glycerol content.

Formulation

Lyophilized with 0.1% glycerol.

Solubility

It is recommended to reconstitute the lyophilized Antigen-85B in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Ag85B although stable room temperature for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Usage

Bio-world products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.