Non-Metastatic Cells 4 Human, Recombinant

Synonyms

Non-metastatic cells 4, nm23-H4, NM23H4, Nucleoside diphosphate kinase D, NDK, NDPKD, NDP kinase mitochondrial, EC 2.7.4.6, NDPK-D.

NME4 is a member of the NDK family. NME4 are ubiquitous enzymes which catalyze transfer of gamma-phosphates, using a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family that include NME4. NME4 has a key part in the synthesis of nucleoside triphosphates other than ATP.

Description

NME4 Human Recombinant fused with a 210 amino acid His tag at N-terminus produced in E. coli is a single, non-glycosylated, polypeptide chain containing 176 amino acids (33-187a.a.) and having a molecular mass of 19.6kDa. The NME4 is purified by proprietary chromatographic techniques.

Source

Escherichia coli

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The NME4 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 40% glycerol and 0.2M NaCl.

Stability

NME4 should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MPSWTRERTL VAVKPDGVQR RLVGDVIQRF ERRGFTLVGM KMLQAPESVL AEHYQDLRRK PFYPALIRYM SSGPVVAMVW EGYNVVRASR AMIGHTDSAE AAPGTIRGDF SVHISRNVIH ASDSVEGAQR EIQLWFQSSE LVSWADGGQH SSIHPA

Usage

Bio-world products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.