Ovine Growth Hormone (Recombinant)

Recombinant Ovine(sheep) Growth Hormone

Source: CHO cells

Background: A polypeptide that is secreted by the adenohypophysis . Growth hormone, also known as somatotropin, stimulates mitosis, cell differentiation and cell growth. Species-specific growth hormones have been synthesized. GH augments the cytolytic activity of T-cells, antibody synthesis, and granulocyte differentiation induced by GM-CSF . GH also enhances production of TNF-alpha , generation of superoxide anions from peritoneal macrophages, and natural killer activity.

Description: Recombinant Ovine Growth Hormone produced in E.Coli is a single, non-glycosylated polypeptide chain containing 200 amino acids and having a molecular mass of 22015 Dalton. Recombinant Ovine GH is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Ovine-GH was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.

Solubility: It is recommended to reconstitute the lyophilized Ovine-GH in sterile 18MO-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized Ovine-GH although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Ovine-GH should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Phe-Pro-Ala.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: Ovine Growth Hormone is fully biologically active when compared to standard. The activity as determined by the dose-dependant stimulation of the proliferation FDCP13B9 cells.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of roGH.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.7 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Ovine-GH as a Reference Standard.

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.