Ovine Prolactin Soluble Receptor (Recombinant)

Recombinant Salmon/Trout Insulin Like Growth Factor-I

Source: E.Coli

Background: IGF is a well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on Somatotropin. It is believed to be mainly active in adults in contrast to Insulin like Growth Factor 2, which is a major fetal growth factor.

Description: Recombinant Salmon/Trout IGF-I produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7704 Dalton. IGF-I is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Recombinant IGF-1 was lyophilized after extensive dialysis against 100mM acetic acid.

Solubility: It is recommended to reconstitute the lyophilized IGF-1 in sterile 18MO-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized IGF-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution IGF-1 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: IGF-I is fully biologically active when compared to standards. The ED50, calculated by the Stimulation of protein synthesis in rat L6 myoblasts was found to be less then 50 ng/ml.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of IGF-I.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC, using a standard solution of Salmon IGF-I as a Reference Standard.

References: Cao Q. et al. (1989) Mol. Endocrinol. 3, 2005-2010 Shamblott M. J. & Chen T.T. (1992) Proc. Natl. Acad. Sci. 89, 8913-8917 Moriyama S. et al. (1995) Gen. Comp. Endocrinol. 99, 221-229

Usage: BioWORLD\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.