Polyphospate Kinase (Recombinant)

Polyphosphate Kinase (PPK)

Source: Propionibacterium shermanii

Background: PPK catalyzes the reversible transfer of phosphate between polyphosphate and ATP The phosphorylation of ADP to ATP by polyphosphate kinase is by a processive mechanism; the phosphorylation occurs without release of the polymer from the enzyme prior to termination of the reaction.

Description: Polyphosphate Kinase purified circa 10 fold . Free of all Polyphosphate Glucokinase activity. Native MW = 83 kD (monomer)

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: The protein was lyophilized from PPK solution containing 11.54 U/ml of PPK activity, 10.3 mg/ml total protein, 100mM potassium phosphate pH 6.8 and 25mM sodium polyphosphate.

Stability: Lyophilized rPPK although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution rHuGH should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Solubility: The contents of the vial can be reconstituted in 1-10 ml deionized water.

Unit Definition: The amount of PPK required to convert 1 mole ADP to ATP per minute at pH 7.5, using polyphosphate as phosphate donor.

Specific Activity: 1.12U/mg.

Required Cofactors: The reaction requires Mg2+

Usage: BioWORLD\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.