ProMatrix MetalloProteinase-7 (Recombinant)

Recombinant ProMatrix Metalloproteinase-7(proMMP-7)

Source: E.Coli

Background: Matrix metalloproteinase-7 (MMP-7) also known as matrilysin and PUMP (EC 3.4.24.23) cleaves a number of substrates including collagen types IV and X, elastin, fibronectin, gelatin, laminin and proteoglycans. MMP-7 is closely related to the stromelysin family members but is encoded by a different gene. MMP-7 is the smallest of all the MMPs consisting of a pro-peptide domain and a catalytic domain. It lacks the hemopexin-like domain common to other members of the MMPs. MMP-7 is secreted as a 28 kDa proenzyme and can be activated in vitro by organomercurials and trypsin and in vivo by MMP-3 to a 18 kDa active MMP-7 enzyme. Once activated, MMP-7 can activate pro-MMP-1 and pro-MMP-9 but not pro-MMP-2. MMP-7 is widely expressed having been reported in elevated levels in cycling endometrium as well as in colorectal cancers and adenomas, hepatocellular carcinomas, rectal carcinomas, and approximately 50% of gliomas.

Physical Appearance: Sterile filtered clear solution.

Formulation: Recombinant ProMMP-7 ,at a concentration of 1mg/ml, 25mM Tris-HCl (pH 7.5), 150mM NaCl, 5mM CaCl2, 0.01% Brij-35 and 0.02% NaN3.

Stability: ProMatrilysin, although stable at 4C for 3 weeks, should be stored desiccated below -18 C. Please avoid freeze-thaw cycles.

Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: The specific activity was found to be 1400 IU/mg.

Unit definition: One unit is defined as the digestion of 1 g Azocoll/min at 37 C.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Recombinant ProMMP-7 .

Usage: BioWORLD\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.