Rabbit Prolactin Soluble Receptor (Recombinant)

Recombinant Rabbit Prolactin receptor ECD (rbRPRLR-ECD)

Source: E.Coli

Description: Recombinant rRPRL-R-ECD produced in E.Coli is a non-glycosylated, Polypeptide chain containing 207 amino acids and having a molecular mass of 23972 Dalton. The rRPRLR-ECD is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.

Solubility: It is recommended to reconstitute the lyophilized rbRPRLR-ECD in sterile 18M-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized rbRPRLR-ECD although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rbRPRLR-ECD should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Purity: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Lys-Pro-Phe-Ile.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: rbRPRLr-ECD is fully biologically active: its activity is determined by the dose-dependant inhibition of PRL-stimuled proliferation of Nb2 cells and by high affinity binding of oPLR and other lactogenic hormones. Refs: 1) Bignon et al. (1994) JBC 269; 3318-24 2) Gertler et al. (1996) JBC 271; 24482-91.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of rbRPRLR-ECD.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 2.63 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of rbRPRLr-ECD as a Reference Standard.

Usage: BioWORLD\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.