Rabbit Skeletal Muscle Troponin-C (Recombinant)

Recombinant Rabbit Cardiac Troponin-C

Source: E.Coli

Background: Cardiac troponin C belongs to the EF-hand superfamily of calcium-binding proteins and plays an essential role in the regulation of muscle contraction and relaxation.

Description: Recombinant Rabbit TNC produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 18,020 Dalton. Recombinant Cardiac Troponin-C is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered lyophilized powder.

Formulation: Lyophilized from (1mg/ml) solution containing 150mM NaCl, 10mM sodium phosphate, 0.5mM EDTA and 0.02% NaN3, (pH 7.0).

Solubility: It is recommended to reconstitute the lyophilized TNC in sterile 18M -cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized TNC although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution TNC should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Rabbit TNC.

Protein content: Protein quantitation was carried out by: 1-10 mg/ml Bradford assay vs. BSA

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.