RAP1A, Member RAS Oncogene Family Human, Recombinant

Synonyms

KREV1, G-22K, C21KG, RAP1A, RAP1, Ras-related protein Rap-1A, GTP-binding protein smg p21A, Ras-related protein Krev-1.

RAP1A is part of the family of RAS-related proteins which possess 50% amino acid identity with the classical RAS proteins and have various structural characteristics in common. RAP proteins differ from RAS proteins in their 61st amino acid, glutamine in RAS is replaced by threonine in RAP proteins. RAP1A induces morphological reversion of a cell line transformed by a Ras oncogene and counteracts the mitogenic function of Ras, since it can interact with Ras GAPs and RAF in a competitive manner.

Description

RAP1A Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing 201 amino acids (1-181 a.a.) and having a molecular mass of 22.8 kDa. The RAP1A is fused to 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Source

E. coli

Physical Appearance

Sterile filtered colorless solution.

Formulation

RAP1A Human solution containing 20mM Tris HCL pH-7.5, 5mM DTT, 0.2M NaCl & 40% glycerol.

Stability

RAP1A Human although stable at 4°C for 1 week, should be stored desiccated below -18°C. Please prevent freeze thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDCQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTEDVP MILVGNKCDL EDERVVGKEQ GQNLARQWCN CAFLESSAKS KINVNEIFYD LVRQINRKTP VEKKKPKKKS C

Usage

Bio-world products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

RAP1B Human, RAP2A Human