Rat GRO-beta/MIP-2 (CXCL2), (Recombinant)

Recombinant Rat GRO-Beta (CXCL2)

Source: E.Coli

Background: Three MGSA/GRO genes encode 3 highly related chemokines, MGSA/GRO alpha, beta and gamma. All 3 MGSA/GRO proteins bind to the same receptors, but with differing affinities, and stimulate a number of biological responses including chemotaxis, angiogenesis, and growth regulation.

Description: Recombinant Rat GRO-beta (MIP-2A) produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 73 amino acids and having a molecular mass of 7923 Dalton. Rat GRO-b is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: The protein was lyophilized with no additives.

Solubility: It is recommended to reconstitute the lyophilized Rat GRO-beta in sterile 18MO-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized CXCL2 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution rat GRO-beta should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Val-Val-Val-Ala-Ser.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: The Biological activity is calculated by its ability to chemoattract total rat neutrophils cells using 10.0-100.0 ng/ml.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Rat GRO-beta .

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.015 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of CXCL2 as a Reference Standard.

Usage: BioWORLD\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.