Rat Interleukin-1 beta (Recombinant)

Recombinant Rat Interleukin-1 beta

Source: E.Coli

Background: A soluble factor produced by monocytes, macrophages, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. IL-1 consists of two distinct forms, IL-1 alpha and IL-1 beta which perform the same functions but are distinct proteins. The synthesis of IL-1 can be induced by other cytokines including TNF-alpha , IFN-alpha , IFN-beta and IFN-g and also by bacterial endotoxins , viruses, mitogens, and antigens. In human skin fibroblasts IL-1 alpha and TNF-alpha induce the synthesis of IL-1beta . In pheochromocytoma cells, NGF induces the synthesis of IL-1.

Description: Recombinant Rat Interleukin-1 beta produced in E.Coli is a non-glycosylated, Polypeptide chain containing 153 amino acids and having a molecular mass of 17415.82 Dalton. The Recombinant Rat IL-1b is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing 10mM sodium citrate pH=6.5.

Solubility: It is recommended to reconstitute the lyophilized Rat Interleukin-1b in sterile 18MO-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized Rat IL-1 beta although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution IL-1 beta should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Val-Pro-Ile-Arg.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: The ED50 as determined by the dose-dependant stimulation of murine D10S cells is < 0.1 ng/ml, corresponding to a Specific Activity of 1 x 107 IU/mg.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Recombinant Rat Interleukin-1 beta.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.558 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of Rat IL-1b as a Reference Standard.

Usage: BioWORLD\\\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.