Rat Macrophage Inflammatory Protein-1 alpha (CCL3) (Recombinant)

Recombinant Rat Macrophage Inflammatory Protein-1 alpha (MIP-1a)

Source: E.Coli

Description: Recombinant Rat MIP-1a produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7853 Dalton. Recombinant Rat MIP-1a is purified by proprietary chromatographic techniques

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: The protein was lyophilized from 1mg/ml solution containing no additives.

Solubility: It is recommended to reconstitute the lyophilized Rat MIP-1alpha in sterile 18MO-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized MIP-1alpha although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution MIP-1 alpha should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Tyr-Gly-Ala.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: Recombinant Rat MIP-1a is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract of rat peritoneal macrophages using a conc. of 60 ng/ml.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Recombinant MIP-1alpha.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.38 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of MIP-1 alpha as a Reference Standard.

Usage: BioWORLD\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.